TaqMan Performance Guarantee* ensures the assay will work as described or we’ll replace itĮach TaqMan SNP Genotyping Assay includes two differentially labeled, allele-specific TaqMan MGB probes and a PCR primer pair that uniquely amplify and provide unmatched specificity for the allele of interest.Gold-standard chemistry backed by more than 40,000 publications and over 300 patents.Flexible formats, including single tubes 48-, 96-, and 384-well plates and OpenArray plates, to accommodate any throughput and number of targets and samples.Functional quality testing with 20 gDNA samples for each human assay.
#ALLELEID MGB ALLELE PROBE GENOTYPING MAC OS X#
AlleleID 6.01 Released AlleleID now offers compatibility with Leopard, Mac OS X 10.5. Using AlleleID you can design NEAT probes, both for copy number detection and mutation studies. NEAT, Non-Enzymatic Amplification Technology, is a genomic testing platform. A graphical representation of fluorescence and normalized reporter signal (DeltaRn) values demonstrated that HLA-B27 positive and HLA-B27 negative samples formed two tight clusters making it possible to clearly differentiate between HLA-B27 positive and negative samples. AlleleID can now design highly specific probes for NEAT assays. Serology and our TaqMan assay gave identical results in all cases and no false positive or negative results were observed. We tested this method on 150 subjects (41 HLA-B27 positive and 109 HLA-B27 negative) who underwent routine HLA-B27 serological testing by flow cytometry (FC). Moreover, the use of a non-fluorescent quencher (NFQ) attached to the MGB probe improves the efficiency of fluorescence quenching, thus providing a very low fluorescent background. The MGB increases the melting temperature (T(m)) of the probe, allowing the use of shorter probes. We describe a real-time PCR method for the detection of the HLA-B27 allele using sequence-specific primers (SSP) combined with a fluorogenic MGB probe (minor groove binder probe), a novel type of TaqMan probe. Therefore, several more accurate molecular methods have been developed for HLA-B27 genotyping. contig analysis and design of sequencing primers AlleleID and Beacon. As typing for HLA-B27 is routinely performed by serological methods, false-positive results can be generated. In an embodiment of the invention, the TaqMan MGB probes can be labelled with.
With ClustalW multiple sequence alignment at its core, AlleleID can be used to design species identification/cross species probes for microarrays or real time PCR including SYBR Green, TaqMan MGB, TaqMan probes, Molecular Beacons and real time PCR primers. AlleleID can help you determine the species of a bacteria even if you have a partial oligonucleotide sequence of a gene. AlleleID is a comprehensive desktop tool designed to address the challenges of bacterial identification, pathogen detection or species identification. HLA-B27 is strongly associated with ankylosing spondylitis (AS). Assay Design for Bacterial Identification and More.
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